Smyth W Franklin
School of Biomedical Sciences, University of Ulster, Coleraine, UK.
Electrophoresis. 2006 Jun;27(11):2051-62. doi: 10.1002/elps.200500524.
This review considers applications in 2004-2005 of capillary electrophoresis-electrospray ionisation-mass spectrometry (CE-ESI-MS) to the detection and determination of small molecular mass drug molecules, taken from the Web of Knowledge database. The molecules of small molecular mass less than 1000 Da are chosen according to selected structural classes in which they give ESI signals primarily as M + H ions. These structural classes are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, 1,4-benzodiazepines, other heterocyclic hypnotics, steroids, bioactive compounds containing phenolic groups, and miscellaneous molecules. Details are given on the fragmentations, where available, that these ionic species exhibit in-source and in ion-trap, triple quadrupole and time-of flight mass spectrometers. The review then gives a critical evaluation of these recent CE-ESI-MS analytical methods for the detection and determination of these small molecular mass drug molecules. Analytical information on, for example, sample concentration techniques, CE separation conditions, recoveries from biological media and limits of detection are provided.
本综述探讨了2004 - 2005年毛细管电泳 - 电喷雾电离 - 质谱联用技术（CE - ESI - MS）在小分子质量药物分子检测与测定中的应用，相关内容取自《科学网》数据库。根据选定的结构类别选取了分子量小于1000 Da的小分子质量分子，这些分子主要以M + H离子形式给出电喷雾电离信号。这些结构类别包括含胺侧链的药物、含氮饱和环结构的药物、1,4 - 苯二氮䓬类、其他杂环催眠药、甾体类、含酚基的生物活性化合物以及其他杂类分子。文中还给出了这些离子物种在源内以及在离子阱、三重四极杆和飞行时间质谱仪中所呈现的碎裂细节（如有）。随后，本综述对这些用于检测和测定小分子质量药物分子的最新CE - ESI - MS分析方法进行了批判性评估。提供了例如样品浓缩技术、毛细管电泳分离条件、从生物介质中的回收率以及检测限等分析信息。
