Hamzeh N, Koohi Habibi M, Mosahebi G, Ghazanfari K
Dept. of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Iran.
Commun Agric Appl Biol Sci. 2005;70(3):417-22.
During two growing seasons in years of 2003 and 2004 potato and tobacco of virus infected plants were collected from fields in Tehran (Damavand) and Mazandaran (Behshahr) provinces. Serological methods of TAS-ELISA and DIBA were performed by using PVY antiserum (DSMZ - Plant Virus Collection; Germany) but only PVY was detected. The strain of samples was determined by using MAb of potato virus Y (AS-0403/1; DSMZ; Germany). The molecular weight of the virus coat protein was approximately 34 kDa in SDS-PAGE and Western blotting. Total RNA was extracted for RT-PCR. Immunocapture RT-PCR and RT-PCR products were 974 bp by using specific primers of PVY. IC-RT-PCR has given the best results.
在2003年和2004年的两个生长季节，从德黑兰（达马万德）和马赞德兰（贝赫沙赫尔）省的田间收集了感染病毒的马铃薯和烟草植株。使用PVY抗血清（德国DSMZ - 植物病毒保藏中心）进行了TAS-ELISA和DIBA血清学方法检测，但仅检测到PVY。使用马铃薯Y病毒单克隆抗体（AS-0403/1；德国DSMZ）确定样品的毒株。在SDS-PAGE和蛋白质印迹法中，病毒外壳蛋白的分子量约为34 kDa。提取总RNA用于RT-PCR。使用PVY特异性引物，免疫捕获RT-PCR和RT-PCR产物为974 bp。IC-RT-PCR给出了最佳结果。
