Kazinczi G, Takács A P, Horváth J, Gáborjányi R, Béres I
Office for Academy Research Groups Attached to Universities and Other Institutions, Virology Group, University of Veszprém, Georgikon Faculty of Agricultural Sciences, H-8361 Keszthely, P.O.Box 71, Hungary.
Commun Agric Appl Biol Sci. 2005;70(3):489-91.
The aim of our investigations was to study the susceptibility of 22 weed species to Pepino mosaic virus (PepMV). Seven plants of each species were mechanically inoculated at 4-6 leaf stage with PepMV in a vector free virological glasshouse. Inoculated plants were tested on the basis of symptoms, by DAS ELISA serological method and back inoculation. Among the 22 weed species, only some Solanum ones (S. aethiopicum, S. dulcamara, S. luteum, S. nigrum) were susceptible to PepMV infection. All of them--except S. aethiopicum--belong to Hungarian weed flora, therefore these weeds--occurring mainly in tomato ecosystems--may play important role in the epidemiology of PepMV. Other 18 weed species seemed to be resistant to PepMV, and on the basis of back inoculation not even latent infection has been observed.
我们研究的目的是研究22种杂草对番茄花叶病毒（PepMV）的易感性。在无载体的病毒学温室中，在4-6叶期对每个物种的7株植物进行PepMV的机械接种。对接种的植物根据症状、通过双抗夹心酶联免疫吸附测定（DAS ELISA）血清学方法和回接进行检测。在这22种杂草中，只有一些茄属植物（非洲茄、欧白英、黄果茄、龙葵）易受PepMV感染。除非洲茄外，所有这些植物都属于匈牙利杂草区系，因此这些主要出现在番茄生态系统中的杂草可能在PepMV的流行病学中起重要作用。其他18种杂草似乎对PepMV具有抗性，并且根据回接结果，甚至未观察到潜伏感染。
