[宫颈上皮内瘤变中抑癌基因的异常甲基化与等位基因失衡]

[Aberrant methylation of tumor suppressor genes and allelic imbalance in cervical intraepitelial neoplasia].

作者信息

Kekeeva T V, Zhevlova A I, Podisov Iu I, Solov'eva Iu V, Zaletataev D V, Nemtsova M V

出版信息

Mol Biol (Mosk). 2006 Mar-Apr;40(2):224-30.

Abstract

We analysed 42 high-grade CIN or CIN3 samples, 42 nondysplasia tissues adjacent to CIN3. 35 smears from women without gynecological pathology were also evaluated. Methylation status of six genes (p16, MLH1, HIC1, MGMT, N33 and RB1) was determined using methylation-sensitive PCR. There is some insignificant level of methylation determined in normal smears. Methylation percentages of the genes in CIN3 were: p16, 58%; MLH1, 51%; HIC1, 84%; N33, 27%. Methylation percentages of the genes in nondysplasia adjacent tissues were also high. There is no significant difference in methylation frequencies of MGMT and RB1 determined between dysplasia and control. We identified allelic imbalance at chromosomes 5q11-q14 and 13q14 in 21% cases (9/42). The incidence of LOH was investigated in 7% (3/42) cases at region 13q14.

摘要

我们分析了42个高级别CIN或CIN3样本、42个与CIN3相邻的无发育异常组织。还评估了35名无妇科病理的女性的涂片。使用甲基化敏感PCR测定了六个基因（p16、MLH1、HIC1、MGMT、N33和RB1）的甲基化状态。在正常涂片中检测到一些无显著意义的甲基化水平。CIN3中各基因的甲基化百分比分别为：p16，58%；MLH1，51%；HIC1，84%；N33，27%。无发育异常的相邻组织中各基因的甲基化百分比也很高。在发育异常组织和对照之间，MGMT和RB1的甲基化频率没有显著差异。我们在21%的病例（9/42）中发现了5号染色体q11-q14和13号染色体q14的等位基因失衡。在13号染色体q14区域，对7%（3/42）的病例进行了杂合性缺失发生率的研究。