Panin L E, Tuzikov F V, Tuzikova N A, Pliakov L M
Mol Biol (Mosk). 2006 Mar-Apr;40(2):300-9.
On primary culture of hepatocytes it is shown, that a complex cortisol-apolipoprotein A-I did not change rate of biosynthesis DNA and protein, whereas the complex tetrahydrocortisol-apolipoprotein A-I (THC-apoA-I) essentially raised rate of incorporation 3H-thymidine in DNA and 14C-leucine into protein. By a method of small-angle X-ray scattering it is shown, that appreciable interaction with eukariotic DNA is marked only in case of use of a complex THC-apoA-I, thus there is local fusion of DNA. The most probable region of interaction of the given complex with DNA is repetition (GCC)n the type, included in structure of many genes eukariot, including the human. It is synthesized oligonucleotid (duplex) of this type. It is shown, that at his interaction with complex THC-apoA-I there is a formation of more difficult complex, which breaks up with formation of complementary chains of oligonucleotides. The last also enter interaction with complex THC-apoA-I. It is given of kinetic this multiphasic process. Interaction of a complex cortisol-anoA-I with a duplex is less specific and does not result reduce in decay of the duplex and in formation of complementary oligonucleotides.
在肝细胞原代培养中发现，皮质醇 - 载脂蛋白A-I复合物不会改变DNA和蛋白质的生物合成速率，而四氢皮质醇 - 载脂蛋白A-I复合物（THC-apoA-I）则显著提高了3H-胸腺嘧啶核苷掺入DNA以及14C-亮氨酸掺入蛋白质的速率。通过小角X射线散射法表明，仅在使用THC-apoA-I复合物时才会与真核生物DNA发生明显相互作用，此时会出现DNA的局部融合。该复合物与DNA相互作用最可能的区域是（GCC）n型重复序列，其包含在许多真核生物基因（包括人类基因）的结构中。合成了这种类型的寡核苷酸（双链体）。结果表明，在其与THC-apoA-I复合物相互作用时会形成更复杂的复合物，该复合物会分解形成寡核苷酸的互补链。后者也会与THC-apoA-I复合物发生相互作用。给出了这个多相过程的动力学情况。皮质醇 - anoA-I复合物与双链体的相互作用特异性较低，不会导致双链体的降解减少以及互补寡核苷酸的形成。
