Grokhovskiĭ S L
Mol Biol (Mosk). 2006 Mar-Apr;40(2):317-25.
Cleavages of double-stranded DNA fragments of known base pair sequence upon ultrasound irradiation at 22 and 44 kHz were studied by gel electrophoresis. The cleavage rate is found to be strongly dependent on the DNA fragment length, pH, temperature and ionic strength of the solution under study. The cleavage of double-stranded DNA occurs predominantly at sites containing alternating 5'-CpG-3' sequences. The breakage of phosphatediester bond takes place between C and G in such a way that phosphate group at the 5'-end of the guanine residue remain intact. The cleavage rate at a given DNA site is found to depend on base pair sequences at adjacent sites. Distinctly different cleavage patterns are observed when free DNA and DNA complexes with cys-diammine-Pt-bridged bis-netropsin were irradiated by ultrasound. The observed effect can be attributed to local DNA conformation changes induced upon complex formation between ligand and DNA.
通过凝胶电泳研究了在22千赫和44千赫超声辐照下已知碱基对序列的双链DNA片段的切割情况。发现切割速率强烈依赖于所研究溶液中DNA片段的长度、pH值、温度和离子强度。双链DNA的切割主要发生在含有交替的5'-CpG-3'序列的位点。磷酸二酯键在C和G之间断裂，使得鸟嘌呤残基5'-端的磷酸基团保持完整。发现在给定的DNA位点的切割速率取决于相邻位点的碱基对序列。当游离DNA和与半胱氨酸-二胺-铂桥联双奈替普辛形成的DNA复合物受到超声辐照时，观察到明显不同的切割模式。观察到的效应可归因于配体与DNA形成复合物时诱导的局部DNA构象变化。
