Sidorova M V, Molokoedov A S, Az'muko A A, Aref'eva T I, Melekhov M G, Kukhtina N B, Krasnikova T L, Bespalova Zh D, Bushuev V N
Bioorg Khim. 2006 Mar-Apr;32(2):161-8.
The retro-enantio analogue of peptide 66-77 of the chemokine MCP-1 and two hexapeptide fragments 66-71 and 72-77 of the C-terminal sequence of this protein were synthesized using the Fmoc strategy of solid phase peptide synthesis. The effect of the synthetic peptides upon the MCP-1-stimulated migration of THP-1 mononuclear cells was studied in vitro. The activity of the retro-enantio analogue was found to be comparable with that of the initial peptide 66-77: both peptides inhibit the migration of monocytes and granulocytes into inflammation zones of experimental animals.
使用固相肽合成的Fmoc策略合成了趋化因子MCP-1的肽66-77的反向对映体类似物以及该蛋白C端序列的两个六肽片段66-71和72-77。体外研究了合成肽对MCP-1刺激的THP-1单核细胞迁移的影响。发现反向对映体类似物的活性与初始肽66-77的活性相当：两种肽均抑制单核细胞和粒细胞向实验动物炎症区域的迁移。
