Antal T K, Volgusheva A A, Kukarskikh G P, Krendeleva T E, Tusov V B, Rubin A B
Biofizika. 2006 Mar-Apr;51(2):292-8.
Modulated fluorometry (PAM) was applied for probing the photosynthesis in cells of C. reinhardtii during sulfur deprivation. A significant (up to a fourfold) increase in chlorophyll fluorescence yield (parameters F(o) and F(m)) normalized to chlorophyll concentration was shown for deprived cells. An analysis of nonphotochemical quenching of chlorophyll fluorescence indicated a considerable modification of the energy deactivation pathways in PS II of sulfur-deprived cells. Thus, starved cells exhibited a lower deltapH-dependent quenching of excited states and a higher thermal dissipation of excess light energy in reaction centers of PS II, as well as the transition of the photosynthetic apparatus primarily to state 2. However, these changes cannot cause the elevation of chlorophyll fluorescence in the cells under sulfur limitation. The phenomenon observed may be due to a partial dissociation of light-harvesting complexes from reaction centers of PS II and/or dysfunction of the dissipative cycle in PS II with cytochrome b559 as an intermediate.
调制荧光法（PAM）被用于探测莱茵衣藻细胞在硫缺乏期间的光合作用。相对于叶绿素浓度，缺硫细胞的叶绿素荧光产量（参数F(o)和F(m)）显著增加（高达四倍）。对叶绿素荧光非光化学猝灭的分析表明，硫缺乏细胞的PS II中能量失活途径有相当大的改变。因此，饥饿细胞在PS II反应中心表现出较低的依赖ΔpH的激发态猝灭和较高的过剩光能热耗散，以及光合机构主要向状态2的转变。然而，这些变化不会导致硫限制条件下细胞中叶绿素荧光的升高。观察到的现象可能是由于光捕获复合物与PS II反应中心的部分解离和/或PS II中以细胞色素b559为中间体的耗散循环功能障碍。
