[核磁共振光谱法在氧化葡萄糖杆菌对山梨醇和葡萄糖转化研究中的应用]
[Use of NMR spectroscopy in studies of sorbitol and glucose transformation by Gluconobacter oxydans].
作者信息
Kitova a E, Reshetilov A N, Kutyshenko V P, Kutyshenko A V
出版信息
Biofizika. 2006 Mar-Apr;51(2):306-9.
PMID:16637338
Abstract
NMR spectroscopy was applied for studying the products of glucose and sorbitol oxidation by cells of Gluconobacter oxydans. An analysis of 1H NMR spectra showed that the transformation of glucose results in the formation of diketogluconic acid, and sorbitol is oxidized to sorbose. In the 32P NMR spectra, only a signal of inorganic phosphate was detected, which accumulated in the medium as a result of cell lysis.
摘要
应用核磁共振光谱法研究氧化葡萄糖杆菌细胞对葡萄糖和山梨醇的氧化产物。对¹H核磁共振光谱的分析表明，葡萄糖的转化会导致二酮葡萄糖酸的形成，而山梨醇被氧化为山梨糖。在³²P核磁共振光谱中，仅检测到无机磷酸盐的信号，其是细胞裂解后在培养基中积累的。
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