Cunha Sara C, Amaral Joana S, Fernandes José O, Oliveira M Beatriz P P
REQUIMTE, Serviço de Bromatologia, Faculdade de Farmacia, Universidade do Porto, Portugal.
J Agric Food Chem. 2006 May 3;54(9):3351-6. doi: 10.1021/jf053102n.
Three different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils. To support the validity of the method, the parameters evaluated were linearity, detection limits, repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six identified compounds: alpha-T, beta-T, gamma-T, delta-T, alpha-T3, and gamma-T3. Alpha-tocopherol (alpha-T) was the main vitamin E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol composition of the olive oils under evaluation.
串联的荧光和二极管阵列、紫外以及蒸发光散射检测系统。使用荧光检测器获得了最佳结果，该检测器成功应用于对18个葡萄牙橄榄油样品中生育酚和生育三烯酚的定量分析。为支持该方法的有效性，评估的参数包括线性、检测限、重复性和回收率。所有研究样品均显示出相似的定性图谱，鉴定出六种化合物：α-生育酚（α-T）、β-生育酚（β-T）、γ-生育酚（γ-T）、δ-生育酚（δ-T）、α-生育三烯酚（α-T3）和γ-生育三烯酚（γ-T3）。α-生育酚（α-T）是所有样品中的主要维生素E异构体，含量范围为93至260毫克/千克。生育酚和生育三烯酚的总量范围为100至270毫克/千克。地理来源似乎并未影响所评估橄榄油中生育酚和生育三烯酚的组成。
