Panfili Gianfranco, Fratianni Alessandra, Irano Mario
DISTAAM, Università degli Studi del Molise, via De Sanctis, 86100 Campobasso, Italy.
J Agric Food Chem. 2003 Jul 2;51(14):3940-4. doi: 10.1021/jf030009v.
The eight vitamers of vitamin E (alpha-, beta-, gamma-, and delta-tocopherols and -tocotrienols) have different antioxidant and biological activities and have different distributions in foods. Some cereals, especially oat, rye, and barley, are good sources of tocotrienols. A fast procedure for the determination of tocopherols and tocotrienols (tocols) in cereal foods was developed. It involves sample saponification and extraction followed by normal phase high-performance liquid chromatography (HPLC). The results have been compared with those found by direct extraction without saponification. The method is sensitive and selective enough to be tested on a wide variety of cereal samples. The highest tocol levels were found in soft wheat and barley ( approximately 75 mg/kg of dry weight). beta-Tocotrienol is the main vitamer found in hulled and dehulled wheats (from 33 to 43 mg/kg of dry weight), gamma-tocopherol predominates in maize (45 mg/kg of dry weight) ), and alpha-tocotrienol predominates in oat and barley (56 and 40 mg/kg of dry weight, respectively).
维生素E的八种维生素形式（α-、β-、γ-和δ-生育酚及生育三烯酚）具有不同的抗氧化和生物活性，在食物中的分布也各不相同。一些谷物，特别是燕麦、黑麦和大麦，是生育三烯酚的良好来源。开发了一种用于测定谷物食品中生育酚和生育三烯酚（生育酚类）的快速方法。该方法包括样品皂化和萃取，然后进行正相高效液相色谱（HPLC）分析。已将结果与未皂化直接萃取法所得结果进行了比较。该方法灵敏度和选择性足够高，可用于多种谷物样品的检测。软质小麦和大麦中的生育酚类含量最高（约75毫克/千克干重）。β-生育三烯酚是带壳和去壳小麦中的主要维生素形式（33至43毫克/千克干重），γ-生育酚在玉米中占主导地位（45毫克/千克干重），而α-生育三烯酚在燕麦和大麦中占主导地位（分别为56和40毫克/千克干重）。
