Ivanova Elena, Müller Frank
Institut für Biologische Informationsverarbeitung 1, Forschungszentrum Jülich, Jülich, Germany.
Vis Neurosci. 2006 Mar-Apr;23(2):143-54. doi: 10.1017/S0952523806232048.
Bipolar cells were recorded in rat retinal slices to study the distribution of hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels. Patch-clamp whole cell measurements were combined with intracellular filling and recorded cells were morphologically identified. HCN channel isoforms HCN1-4 are differentially expressed in bipolar cells. Each bipolar cell type has a characteristic inventory of HCN channels. The combination of HCN channel currents and other voltage-gated currents can be used as a kind of "finger print" to electrophysiologically identify and classify bipolar cell types. Using this approach of combined electrophysiological and morphological classification we could identify a new ON-cone bipolar cell type.
为研究超极化激活的环核苷酸门控（HCN）通道的分布，在大鼠视网膜切片中记录双极细胞。膜片钳全细胞测量与细胞内灌流相结合，并通过形态学鉴定所记录的细胞。HCN通道亚型HCN1 - 4在双极细胞中呈差异表达。每种双极细胞类型都有其特征性的HCN通道组合。HCN通道电流与其他电压门控电流的组合可用作一种“指纹”，以电生理学方式识别和分类双极细胞类型。利用这种电生理学与形态学相结合的分类方法，我们能够识别出一种新的视锥ON双极细胞类型。
