Zhao Yan-Li, Lu Dao-Pei
People Hospital, Institute of Hematology, Peking University, Beijing 100044, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Apr;14(2):208-11.
According to previous clinical experiences of the authors, plumbago zeylanica was effective against acute promyelocytic leukemia (APL). However, its effectiveness has never been proven experimentally or unequivocally clinically. This study was aimed to investigate the effects of plumbagin on the proliferation, cell cycle and apoptosis of APL cell line NB4 Cells. Cell inhibitory rates were detected by MTT colorimetric assay; morphologic changes were observed under light microscope and transmission electron microscope; apoptosis-inducing effects were determined by DNA gel electrophoresis, annexin V/PI double-stained and PI single-stained flow cytometry. The results demonstrated that 2-15 micromol/L plumbagin inhibited the proliferation of NB4 cells in a dose-dependent manner. The morphologic changes of cell apoptosis, such as chromsome condensation and apoptotic body formation, were observed by light microscope and transmission electron microscope. Cell cycle analysis showed that NB4 cells were blocked in G2/M phase of cell cycle. And plumbagin induced annexin V+/PI- cell increase and DNA fragmentation. There was a correlation between cell apoptosis rates and the concentrations of plumbagin in dose-dependent manner (P < 0.05). It is concluded that for the first time the present study shows that plumbagin can inhibit cell proliferation, block cell cycle and induce apoptosis of APL cell line NB4 cells.
根据作者以往的临床经验，白花丹对急性早幼粒细胞白血病（APL）有效。然而，其有效性从未在实验上得到证实，在临床上也未得到明确验证。本研究旨在探讨白花丹醌对APL细胞系NB4细胞增殖、细胞周期及凋亡的影响。采用MTT比色法检测细胞抑制率；在光学显微镜和透射电子显微镜下观察形态学变化；通过DNA凝胶电泳、膜联蛋白V/碘化丙啶双染和碘化丙啶单染流式细胞术测定凋亡诱导作用。结果表明，2 - 15 μmol/L白花丹醌以剂量依赖性方式抑制NB4细胞的增殖。通过光学显微镜和透射电子显微镜观察到细胞凋亡的形态学变化，如染色体浓缩和凋亡小体形成。细胞周期分析表明，NB4细胞被阻滞在细胞周期的G2/M期。白花丹醌诱导膜联蛋白V+/碘化丙啶 - 细胞增加和DNA片段化。细胞凋亡率与白花丹醌浓度之间存在剂量依赖性相关性（P < 0.05）。得出结论，本研究首次表明白花丹醌可抑制APL细胞系NB4细胞的增殖、阻滞细胞周期并诱导其凋亡。
