Ma Li, Xue Yong-Quan, Pan Jin-Lan, He Jun, Wu Ya-Fang, Cen Jian-Nong, Wen Bin-Zhao
Department of Hematology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Apr;14(2):228-31.
This study was aimed to explore the usefulness of multiplex reverse transcription-polymerase chain reaction (multiplex RT-PCR) in detection of fusion genes associated with specific translocations in acute leukemia (AL) patients with normal karyotypes. 37 AL patients with normal karyotypes were analyzed by multiplex RT-PCR. The results showed that 4 types of fusion genes such as PML/RARA, AML1/ETO, CBFbeta/MYH11, BCR/ABL were detected in 8 (21.6%) patients by multiplex RT-PCR. In conclusion, multiplex RT-PCR is useful in detection of fusion genes associated with specific translocations in acute leukemia (AL) with normal karyotypes and it would refine the karyotype analysis. When the normal karyotypes were detected in acute leukemia patients by conventional cytogenetic method, the multiplex RT-PCR should be performed for them.
本研究旨在探讨多重逆转录-聚合酶链反应（多重RT-PCR）在检测核型正常的急性白血病（AL）患者中与特定易位相关的融合基因的实用性。采用多重RT-PCR对37例核型正常的AL患者进行分析。结果显示，通过多重RT-PCR在8例（21.6%）患者中检测到4种融合基因，如PML/RARA、AML1/ETO、CBFbeta/MYH11、BCR/ABL。总之，多重RT-PCR在检测核型正常的急性白血病（AL）中与特定易位相关的融合基因方面是有用的，并且它将完善核型分析。当通过传统细胞遗传学方法在急性白血病患者中检测到正常核型时，应进行多重RT-PCR检测。
