Yuan Hong-Jian, Xu Rui-Rong, Ding Run-Sheng, Jiang Sheng-Hua, Lu Wei
Department of Hematology, Affiliated Hospital, Medical College, Nantong University, Nantong 226001, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Apr;14(2):276-80.
The aim of this study was to investigate the inhibition effect of arsenic sulfide (As2S2) on the growth of in vitro cultured BMMNC from MDS patients and to explore its possible cellular and molecular mechanisms. The apoptosis of MDS cells induced by As2S2 solution of different concentrations were studied with MTT, flow cytometry, and RT-PCR. The results showed that (1) low concentration of As2S2 (0-0.6 mg/L) had no marked inhibition effect on proliferation of MDS cells; (2) after treatment with 1.5-50 mg/L of As2S2, both low risk MDS cells and high risk MDS cells presented typical features of apoptosis with a dose-dependent manner, the expression of bcl-2 mRNA and the ratio of bcl-2/bax obviously decreased after As2S2 treatment (P < 0.05); (3) BMMNC from MDS patients had higher apoptosis ratio than that of BMMNC from control. It is concluded that BMMNC excessive apoptosis exists in MDS patients; low concentration of As2S2 (0-0.6 mg/L) shows no inhibition effect on proliferation of MDS cells; high concentration of As2S2 (1.5-50 mg/L) induces apoptosis of MDS cells.
本研究旨在探讨硫化砷（As2S2）对骨髓增生异常综合征（MDS）患者体外培养的骨髓单个核细胞（BMMNC）生长的抑制作用，并探讨其可能的细胞和分子机制。采用MTT法、流式细胞术及逆转录-聚合酶链反应（RT-PCR）研究不同浓度As2S2溶液诱导MDS细胞凋亡的情况。结果显示：（1）低浓度As2S2（0～0.6mg/L）对MDS细胞增殖无明显抑制作用；（2）1.5～50mg/L As2S2处理后，低危和高危MDS细胞均呈现典型的凋亡特征，且呈剂量依赖性，As2S2处理后bcl-2 mRNA表达及bcl-2/bax比值明显降低（P<0.05）；（3）MDS患者的BMMNC凋亡率高于对照组。结论：MDS患者存在BMMNC过度凋亡；低浓度As2S2（0～0.6mg/L）对MDS细胞增殖无抑制作用；高浓度As2S2（1.5～50mg/L）可诱导MDS细胞凋亡。
