Chen Yan, Lu Zhi-Gang
Department of Blood Transfusion, Zhujiang Hospital, The Souther Medical University, Guangzhou 510282, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Apr;14(2):416-8.
Lyophilization is the best method for preservation of blood cells at present. Lyophilized blood cells could be stored at room temperature for long periods of time, while maintaining a high degree of viability. Lyophilized blood cells facilitates transportation and the costs are low. However, the membrane of blood cells is damaged and viability of blood cells is decreased in lyophilization. Trehalose has been shown to protect membrane, proteins and nucleic acids during freezing and desiccation. Now, researchers present a methor for loading blood cells with trehalose. In this paper, damage effect of lyophilization on blood cells, the mechanism of trehalose protection and the experimental studies on trehalose were reviewed.
冷冻干燥是目前保存血细胞的最佳方法。冻干的血细胞可以在室温下长期保存，同时保持高度的活力。冻干血细胞便于运输且成本较低。然而，在冷冻干燥过程中血细胞的膜会受到损伤，血细胞的活力会降低。海藻糖已被证明在冷冻和干燥过程中能保护膜、蛋白质和核酸。现在，研究人员提出了一种用海藻糖加载血细胞的方法。本文综述了冷冻干燥对血细胞的损伤作用、海藻糖的保护机制以及对海藻糖的实验研究。
