Kawamura M, Kato R, Kikuchi K, Kobayashi K, Ishihara T, Shibata T, Inayama S
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Clin Chim Acta. 1991 Dec 16;203(2-3):225-33. doi: 10.1016/0009-8981(91)90294-m.
We developed a method for measuring the activity of type III collagenolytic enzyme in lung cancer tissue, using as substrate, type III collagen purified from human placenta. In this method [3H]propionate is used for labeling type III collagen, with bacterial collagenase used for making the standard curve. It, therefore, becomes possible to compare type III collagenolytic activity with those of other collagen subtypes (types I and IV). As this method is a fibril assay it is not susceptible to trypsin or other proteases. The average type III collagenolytic enzyme activity was higher in squamous cell carcinoma than in adenocarcinoma, while that of lung cancer tissue exceeded that of normal lung tissue. The activity of type III collagenase increased with the progression from one disease stage to the next.
我们开发了一种用于测量肺癌组织中III型胶原酶活性的方法，该方法使用从人胎盘中纯化的III型胶原作为底物。在该方法中，[3H]丙酸盐用于标记III型胶原，使用细菌胶原酶制作标准曲线。因此，能够将III型胶原酶活性与其他胶原亚型（I型和IV型）的活性进行比较。由于该方法是一种原纤维测定法，所以它不易受胰蛋白酶或其他蛋白酶的影响。鳞状细胞癌中III型胶原酶的平均活性高于腺癌，而肺癌组织的活性超过正常肺组织。III型胶原酶的活性随着疾病阶段从一个阶段发展到下一个阶段而增加。
