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One-electron oxidation of Trolox C and vitamin E by peroxidases.

作者信息

Nakamura M

机构信息

Biophysics Division, Research Institute of Applied Electricity, Hokkaido University.

出版信息

J Biochem. 1991 Oct;110(4):595-7. doi: 10.1093/oxfordjournals.jbchem.a123625.

DOI:10.1093/oxfordjournals.jbchem.a123625
PMID:1663948
Abstract

Direct reactions of peroxidases with Trolox C (a vitamin E analogue) and vitamin E were observed in 50% (v/v) methanol. The kinetic results revealed that the reaction of horseradish peroxidase intermediate Compound II with Trolox C and vitamin E was the rate-determining step, and the rate constants were estimated to be 1.7 x 10(3) and 5.1 x 10(2) M-1.s-1, respectively. Peroxidases catalyzed the one-electron oxidation of Trolox C and vitamin E, and the vitamin E phenoxyl radicals resulting from the peroxidase reactions were detected by continuous-flow ESR spectroscopy.

摘要

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