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使用劣质等级成分预测带壳玉米批次中的黄曲霉毒素和伏马毒素。

Predicting aflatoxin and fumonisin in shelled corn lots using poor-quality grade components.

作者信息

Johansson Anders S, Whitaker Thomas B, Hagler Winston M, Bowman Daryl T, Slate Andy B, Payne Gary

机构信息

U.S. Department of Agriculture-Agricultural Research Service, North Carolina State University, Campus Box 7625, Raleigh, NC 27529-7625, USA.

出版信息

J AOAC Int. 2006 Mar-Apr;89(2):433-40.

PMID:16640290
Abstract

A study was conducted to determine if aflatoxin and fumonisin are concentrated in the poor-quality grade components of shelled corn. Four 1.0 kg test samples were each taken from 23 lots of shelled corn marketed in North Carolina. Inspectors from the Federal Grain Inspection Service divided each test sample into 3 grade components: (1) damaged kernels (DM), (2) broken corn and foreign material (BCFM), and )3) whole kernels (WH). The aflatoxin and fumonisin concentration was measured in each component and a mass balance equation was used to calculate the total concentration of each mycotoxin in each test sample. Averaged across all test samples, the aflatoxin concentrations in the DM, BCFM, and WH components were 1300.3, 455.2, and 37.3 ppb, respectively. Averaged across all test samples, the fumonisin concentrations in the DM, BCFM, and WH components were 148.3, 51.3, and 1.8 ppm, respectively. The DM and BCFM components combined accounted for only 5.0% of the test sample mass, but accounted for 59.8 and 77.5% of the total aflatoxin and fumonisin mass in the test sample, respectively. Both aflatoxin mass (ng) and aflatoxin concentration (ng/g) in the combined DM and BCFM components had high correlations with aflatoxin concentration in the lot. The highest correlation occurred when aflatoxin mass (ng) in the combined DM and BCFM components was related to aflatoxin concentration in the lot (0.964). Similar results were obtained for fumonisin. This study indicated that measuring either aflatoxin or fumonisin in the combined DM and BCFM grade components could be used as a screening method to predict either aflatoxin or fumonisin in a bulk lot of shelled corn.

摘要

开展了一项研究,以确定黄曲霉毒素和伏马毒素是否集中在脱壳玉米的劣质等级成分中。从北卡罗来纳州销售的23批脱壳玉米中各取4个1.0千克的测试样品。联邦谷物检验局的检验员将每个测试样品分为3个等级成分:(1) 破损籽粒 (DM),(2) 碎玉米和杂质 (BCFM),以及 (3) 完整籽粒 (WH)。测量了每个成分中的黄曲霉毒素和伏马毒素浓度,并使用质量平衡方程计算每个测试样品中每种霉菌毒素的总浓度。在所有测试样品中,DM、BCFM和WH成分中的黄曲霉毒素浓度平均分别为1300.3、455.2和37.3 ppb。在所有测试样品中,DM、BCFM和WH成分中的伏马毒素浓度平均分别为148.3、51.3和1.8 ppm。DM和BCFM成分合计仅占测试样品质量的5.0%,但分别占测试样品中黄曲霉毒素和伏马毒素总质量的59.8%和77.5%。DM和BCFM组合成分中的黄曲霉毒素质量(纳克)和黄曲霉毒素浓度(纳克/克)与批次中的黄曲霉毒素浓度均具有高度相关性。当DM和BCFM组合成分中的黄曲霉毒素质量(纳克)与批次中的黄曲霉毒素浓度相关时,相关性最高(0.964)。伏马毒素也得到了类似的结果。这项研究表明,测量DM和BCFM等级组合成分中的黄曲霉毒素或伏马毒素可作为一种筛选方法,用于预测一大批脱壳玉米中的黄曲霉毒素或伏马毒素。

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