van Hengel Arjon J, Capelletti Claudia, Brohee Marcel, Anklam Elke
European Commission, Directorate General Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg 111, B-2440 Geel, Belgium.
J AOAC Int. 2006 Mar-Apr;89(2):462-8.
Results are reported for an interlaboratory validation study of 2 commercially available Iateral flow devices (dipstick tests) designed to detect peanut residues in food matrixes. The test samples used in this study were cookies containing peanuts at 7 different concentrations in the range of 0-30 mg peanuts/kg food matrix. The test samples with sufficient and proven homogeneity were prepared in our laboratory. The analyses of the samples (5 times per level by each laboratory) were performed by 18 laboratories worldwide which submitted a total of 1260 analytical results. One laboratory was found to be an outlier for one of the test kits. In general, both test kits performed well. However, some false-negative results were reported for all matrixes containing < 21 mg peanuts/kg cookie. It must be stressed that the test kits were challenged beyond their cut-off limits (> or = 5 mg/kg, depending on the food matrix). One test kit showed fewer false-negative results, but it led to some false-positive results for the blank materials. The sensitivity of the dipstick tests approaches that achieved with enzyme-linked immunosorbent assays.
报告了一项针对2种市售侧向流动装置（试纸条检测）的实验室间验证研究结果，这些装置旨在检测食品基质中的花生残留。本研究中使用的测试样品是含有花生的饼干，花生浓度在0至30毫克/千克食品基质范围内，共有7种不同浓度。具有充分且已证实均一性的测试样品在我们实验室制备。全球18个实验室对样品进行了分析（每个实验室对每个浓度水平分析5次），共提交了1260个分析结果。发现有一个实验室对其中一种测试试剂盒来说是异常值。总体而言，两种测试试剂盒表现良好。然而，对于所有花生含量低于21毫克/千克饼干的基质，均报告了一些假阴性结果。必须强调的是，测试试剂盒面临的挑战超出了其临界值（≥5毫克/千克，具体取决于食品基质）。一种测试试剂盒显示出较少的假阴性结果，但它对空白材料产生了一些假阳性结果。试纸条检测的灵敏度接近酶联免疫吸附测定所达到的灵敏度。
