一种基于葡萄糖生成的葡萄糖-6-磷酸酶检测方法。

An assay for glucose 6-phosphatase based on the formation of glucose.

作者信息

Bublitz C

机构信息

Dept. of Biochemistry, Biophysics and Genetics, University of Colorado Health Science Center, Denver 80262.

出版信息

Mol Cell Biochem. 1991 Dec 11;108(2):141-4. doi: 10.1007/BF00233118.

DOI:10.1007/BF00233118

PMID:1664041

Abstract

A rapid, convenient method for the assay of glucose 6-phosphatase dependent on the removal of radioactive substrate from radioactive product by Dowex 2 fluoride is described. The enzymatic reaction is stopped by the addition of an ethanolic slurry of the resin. After the tubes are shaken, the radioactivity of glucose in the clarified supernatant layer is measured. The release of glucose is directly proportional to time and enzyme concentration. Detergents do not interfere with the method.

摘要

描述了一种快速、便捷的测定葡萄糖6-磷酸酶的方法，该方法依赖于通过Dowex 2氟化物从放射性产物中去除放射性底物。通过加入树脂的乙醇悬浮液来终止酶促反应。试管振荡后，测量澄清上清液层中葡萄糖的放射性。葡萄糖的释放与时间和酶浓度成正比。去污剂不干扰该方法。