Petrícková Katerina, Hasek Jirí, Benada Oldrich, Petrícek Miroslav
Institute of Microbiology AS CR, Prague 4, Czech Republic.
FEMS Microbiol Lett. 2006 May;258(2):187-93. doi: 10.1111/j.1574-6968.2006.00215.x.
The PkwA protein of the thermophilic actinomycete Thermomonospora curvata has already been reported as the first instance of a WD-40 module-containing protein of prokaryotic origin. This protein is composed of an N-terminal eukaryotic-type protein kinase domain and of seven C-terminal WD-40 repeats. PkwA is a peripheral membrane protein that is linked to the early exponential growth phase of the bacterium. Its intracellular concentrations are extremely low. We have shown that the protein forms high molecular weight complexes and is localized mainly in the tips of the young Thermomonospora vegetative hyphae.
嗜热放线菌弯曲高温单孢菌的PkwA蛋白已被报道为原核生物来源的含WD-40模块蛋白的首个实例。该蛋白由一个N端真核生物型蛋白激酶结构域和七个C端WD-40重复序列组成。PkwA是一种外周膜蛋白，与细菌的早期指数生长期相关。其细胞内浓度极低。我们已经表明，该蛋白形成高分子量复合物，并且主要定位于年轻的弯曲高温单孢菌营养菌丝的顶端。
