Rosconi Federico, Souza Emanuel M, Pedrosa Fabio O, Platero Raúl A, González Cecilia, González Marcela, Batista Silvia, Gill Paul R, Fabiano Elena R
Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable-MEC, Unidad Asociada a Facultad de Ciencias-UdelaR, Montevideo, Uruguay.
FEMS Microbiol Lett. 2006 May;258(2):214-9. doi: 10.1111/j.1574-6968.2006.00218.x.
Herbaspirillum seropedicae Z67 is a nitrogen-fixing bacterium able to colonize the rhizosphere and the interior of several plants. As iron is a key element for nitrogen fixation, we examined the response of this microorganism to iron deficiency under nitrogen fixing conditions. We identified a H. seropedicae exbD gene that was induced in response to iron limitation and is involved in iron homeostasis. We found that an exbD mutant grown in iron-chelated medium is unable to fix nitrogen. Moreover, we provide evidence that expression of the nifH and nifA genes is iron dependent in a H. seropedicae genetic background.
巴西固氮螺菌Z67是一种能够定殖于多种植物根际和内部的固氮细菌。由于铁是固氮的关键元素，我们研究了这种微生物在固氮条件下对缺铁的反应。我们鉴定出一个巴西固氮螺菌exbD基因，它在缺铁条件下被诱导表达，并且参与铁稳态调节。我们发现，在铁螯合培养基中生长的exbD突变体无法固氮。此外，我们提供证据表明，在巴西固氮螺菌的遗传背景下，nifH和nifA基因的表达依赖于铁。
