Teixeira Chirlei G L, Piccoli Andressa, Costa Philipe, Soares Luciano, da Silva-Santos José E
Department of Pharmacy, Universidade da Região de Joinville, Campus Universitário, Joinville, SC, Brazil.
J Pharm Pharmacol. 2006 May;58(5):667-75. doi: 10.1211/jpp.58.5.0012.
Pfaffia glomerata is used in southern American countries against inflammatory diseases. We have explored the ability of a crude hydroalcoholic extract of P. glomerata root (HEPG) to prevent the oedematogenic action of several inflammatory agents in mice. We have examined also the duration of its effects and the mechanisms involved. The oral or intraperitoneal treatment of mice with HEPG (1, 10, 30, 100 or 300 mg kg(-1)) reduced, in a dose-dependent manner, carrageenan-induced paw oedema in the early (1-4 h) and late (48 h) periods. In the early period, the ID50 value (the median dose that caused 50% inhibition) of HEPG was 60.5 (28.5-128.71) and 20.4 (14.8-28.3) mg kg(-1) after oral and intraperitoneal administration, respectively. This effect was still evident when HEPG was administered up to 6 h before carrageenan. HEPG inhibited also paw oedema induced by histamine, serotonin, bradykinin, substance P and bacterial lipopolysaccharide. In addition, oral administration of HEPG increased the levels of nitrate and nitrite in the blood of mice. Further, its anti-oedematogenic action against carrageenan was prevented fully by N(G) nitro-L-arginine-methyl-ester (10 mg kg(-1), s.c.), as well as by methylene blue (20 mg kg(-1), s.c.) or 1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one (2 mg kg(-1), s.c.). The results indicated that stimulation of endogenous production of nitric oxide, followed by soluble guanylate cyclase activation, was implicated in the anti-oedematogenic action of HEPG.
巴西人参在南美洲国家被用于治疗炎症性疾病。我们研究了巴西人参根粗水醇提取物（HEPG）预防几种炎症介质在小鼠中引起水肿作用的能力。我们还研究了其作用持续时间及相关机制。用HEPG（1、10、30、100或300mg kg⁻¹）对小鼠进行口服或腹腔注射治疗，在早期（1 - 4小时）和晚期（48小时）均以剂量依赖方式减轻了角叉菜胶诱导的爪肿胀。在早期，口服和腹腔注射HEPG后的ID50值（引起50%抑制的中位剂量）分别为60.5（28.5 - 128.71）和20.4（14.8 - 28.3）mg kg⁻¹。在角叉菜胶注射前6小时给予HEPG时，这种作用仍然明显。HEPG还抑制了组胺、5-羟色胺、缓激肽、P物质和细菌脂多糖诱导的爪肿胀。此外，口服HEPG可提高小鼠血液中硝酸盐和亚硝酸盐水平。此外，N(G)-硝基-L-精氨酸甲酯（10mg kg⁻¹，皮下注射）、亚甲蓝（20mg kg⁻¹，皮下注射）或1H-[1,2,4]恶二唑并[4,3-α]喹喔啉-1-酮（2mg kg⁻¹，皮下注射）可完全阻止其对角叉菜胶的抗水肿作用。结果表明，内源性一氧化氮产生的刺激，随后可溶性鸟苷酸环化酶的激活，与HEPG的抗水肿作用有关。
