Wang Zhu-jie, Xu Jian
Department of Gynecology, Affiliated Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
Zhonghua Fu Chan Ke Za Zhi. 2006 Mar;41(3):159-61.
To examine the expression of leukemia inhibitory factor (LIF) in chorionic villi of normal early pregnancy, threatened abortion and inevitable abortion, and to assess the effects of LIF on threatened abortion and inevitable abortion.
Expression of LIF in chorionic villi was examined using Western blot. Serum progesterone and human chorionic gonadotropin (hCG) levels were determined using radioimmunoassay. A streptavidin peroxidase conjugated semi-quantitative immunohistochemical assay was used to exam LIF protein expression.
The levels of progesterone and hCG in the normal early pregnancy and threatened abortion groups were (95 +/- 26) nmol/L and (75 +/- 14) kU/L, and (90 +/- 26) nmol/L and (68 +/- 13) kU/L, respectively. The differences were not significant (P > 0.05). The levels of progesterone and hCG in the inevitable group were significantly decreased to (36 +/- 17) nmol/L and (13 +/- 3) kU/L, respectively. The differences when compared to the other groups were statistically significant (P < 0.01). LIF protein expression was observed in all cases and located mainly in the cytoplasm of trophoblast cells. The expression of LIF in inevitable abortion group 0.30 +/- 0.02 was lower than that in other groups (P < 0.01). However, there was no significant difference in the expression of LIF between the normal early pregnancy group and threatened abortion group (P > 0.05).
LIF may play a role in the maintenance of normal pregnancy. Reduction of LIF expression in chorionic villi may induce the decrease of serum progesterone and hCG levels and, ultimately, cause inevitable abortion.
检测白血病抑制因子（LIF）在正常早孕、先兆流产及难免流产绒毛组织中的表达情况，探讨LIF对先兆流产及难免流产的影响。
采用蛋白质免疫印迹法检测绒毛组织中LIF的表达；采用放射免疫法测定血清孕酮及人绒毛膜促性腺激素（hCG）水平；采用链霉亲和素-过氧化物酶偶联的半定量免疫组织化学法检测LIF蛋白表达。
正常早孕组和先兆流产组血清孕酮水平分别为（95±26）nmol/L和（90±26）nmol/L，hCG水平分别为（75±14）kU/L和（68±13）kU/L，两组比较差异无统计学意义（P>0.05）。难免流产组血清孕酮及hCG水平显著降低，分别为（36±17）nmol/L和（13±3）kU/L，与其他两组比较差异有统计学意义（P<0.01）。所有标本均检测到LIF蛋白表达，主要定位于滋养层细胞的细胞质中。难免流产组LIF蛋白表达为0.30±0.02，低于其他两组（P<0.01）；正常早孕组与先兆流产组LIF蛋白表达比较差异无统计学意义（P>0.05）。
LIF可能在维持正常妊娠过程中发挥作用。绒毛组织中LIF表达降低可能导致血清孕酮及hCG水平下降，最终引起难免流产。
