[多氯联苯对苯并(a)芘诱导的HepG2细胞DNA损伤的增强作用]

[Enhancement effect of polychlorinated biphenyl on benzo (a) pyrene-induced DNA damage in HepG2 cells].

作者信息

Zou Ya-ling, Lai Rui-ping, Zhou Li-hong, Li Xiao-yan, Lu Wen-qing

机构信息

Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Mar;40(2):97-100.

PMID:16640906

Abstract

OBJECTIVE

To study the effect of polychlorinated biphenyl, Aroclor1254 on benzo (a) pyrene [B (a) P]-induced DNA damage in HepG2 cells.

METHODS

HepG2 cells were pretreated with Aroclor1254 (11.5, 23 and 46 micromol/L) for 24 hours and then exposed to B (a) P (50 micromol/L). DMSO (10 ml/L) was used as solvent control. Single cell gel electrophoresis (SCGE) and high-performance liquid chromatography-electrochemical detection (HPLC-EC) assays were applied to detect DNA single-strand breaks and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in HepG2 cells, respectively.

RESULTS

Average Oliver tail moment (OTM) and 8-OHdG level in HepG2 cells were significantly increased in B (a) P treated group (1.66 +/- 0.21), (23.31 +/- 6.02) 8-OHdG/10(6)dG than that in solvent control (0.79 +/- 0.15), (12.31 +/- 3.24) 8-OHdG/10(6)dG, respectively. In Aroclor 1254 treated group (11.5, 23.0, 46.0 micromol/L), average OTM were 0.88 +/- 0.20, 1.01 +/- 0.15 and 1.10 +/- 0.16, and 8-OHdG levels were (19.57 +/- 7.57), (22.80 +/- 9.16) and (31.74 +/- 9.25) 8-OHdG/10(6)dG, respectively. A concentration of 46 micromol/L Aroclor1254 caused a significant increase of 8-OHdG level as compared with the solvent control. After pretreatment of HepG2 cells with Aroclor1254 (11.5, 23.0 and 46.0 micromol/L), B (a) P induced more DNA strand breaks (OTM: 2.14 +/- 0.22, 2.43 +/- 0.32 and 2.71 +/- 0.31) and 8-OHdG [(32.50 +/- 3.81), (49.23 +/- 16.66) and (60.36 +/- 18.04) 8-OHdG/10(6)dG] in HepG2 cells than B (a) P alone.

CONCLUSION

Aroclor1254 might enhance B (a) P-induced DNA damage in HepG2 cells, which should imply a synergistic effect of Aroclor1254 on the genotoxicity of B (a) P.

摘要

目的

研究多氯联苯Aroclor1254对苯并（a）芘[B（a）P]诱导的HepG2细胞DNA损伤的影响。

方法

用Aroclor1254（11.5、23和46微摩尔/升）预处理HepG2细胞24小时，然后暴露于B（a）P（50微摩尔/升）。二甲基亚砜（10毫升/升）用作溶剂对照。应用单细胞凝胶电泳（SCGE）和高效液相色谱-电化学检测（HPLC-EC）分析法分别检测HepG2细胞中的DNA单链断裂和8-羟基-2'-脱氧鸟苷（8-OHdG）。

结果

B（a）P处理组HepG2细胞的平均奥利弗尾矩（OTM）和8-OHdG水平显著升高，分别为（1.66±0.21）、（23.31±6.02）8-OHdG/10⁶dG，高于溶剂对照组的（0.79±0.15）、（12.31±3.24）8-OHdG/10⁶dG。在Aroclor 1254处理组（11.5、23.0、46.0微摩尔/升）中，平均OTM分别为0.88±0.20、1.01±0.15和1.10±0.16，8-OHdG水平分别为（19.57±7.57）、（22.80±9.16）和（31.74±9.25）8-OHdG/10⁶dG。与溶剂对照相比，46微摩尔/升的Aroclor1254导致8-OHdG水平显著升高。用Aroclor1254（11.5、23.0和46.0微摩尔/升）预处理HepG2细胞后，B（a）P诱导HepG2细胞产生更多的DNA链断裂（OTM：2.14±0.22、2.43±0.32和2.71±0.31）和8-OHdG[（32.50±3.81）、（49.23±16.66）和（60.36±18.04）8-OHdG/10⁶dG]。