Xu Lina, Sun Liyun, Lu Liqin, Zhong Xiuhui, Ma Yuzhong, Qin Jianhua
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Institute of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Biomed Res Int. 2014;2014:497508. doi: 10.1155/2014/497508. Epub 2014 Mar 10.
Polychlorinated biphenyls (PCBs) are widespread persistent residual environmental pollutants, which affect seriously the growth and reproductive alterations in humans and animals. Aroclor 1254 is a commercial mixture of PCBs. Quercetin is a flavonoid, which acts on estrogen receptors and causes the development of estrogen-related diseases. In this paper, the primary cultured endometrial cells in the pregnant rats were isolated and Aroclor 1254 was used to induce the injured endometrial cells model. The cells were treated with gradient quercetin, the viability of the endometrial cells, the expressions of CYP450, the contents of TNF-α, IL-6, estradiol (E2), and progesterone (P4) were measured. It showed that the viability of the cultured endometrial cells, the expression of CYP1A1 and CYP2B1, and the contents of TNF-α, E2, and IL-6 in the injured endometrial cells increased with the treatment of quercetin. It shows that quercetin has protective effect on the injured endometrial cells in the pregnant rats, this provide a basis on herbal medicine protection for animal reproductive diseases caused by environmental endocrine disruptors.
多氯联苯（PCBs）是广泛存在的持久性环境残留污染物，严重影响人类和动物的生长及生殖功能。Aroclor 1254是一种多氯联苯的商业混合物。槲皮素是一种黄酮类化合物，作用于雌激素受体并引发与雌激素相关的疾病。本文分离了孕鼠的原代培养子宫内膜细胞，并用Aroclor 1254诱导建立受损子宫内膜细胞模型。用梯度槲皮素处理细胞，检测子宫内膜细胞的活力、CYP450的表达、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、雌二醇（E2）和孕酮（P4）的含量。结果显示，经槲皮素处理后，培养的子宫内膜细胞活力、受损子宫内膜细胞中CYP1A1和CYP2B1的表达以及TNF-α、E2和IL-6的含量均增加。表明槲皮素对孕鼠受损子宫内膜细胞具有保护作用，这为环境内分泌干扰物所致动物生殖疾病的草药保护提供了依据。
