[纤维蛋白原对牙龈卟啉单胞菌黏附人口腔上皮细胞的影响]
[Effect of fibrinogen on the adherence of Porphyromonas gingivalis to human oral epithelial cells].
作者信息
Ge Song, Wu Ya-fei, Liu Tian-jia, Zhao Lei, Fei Xiao-lu
机构信息
Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Feb;41(2):69-73.
OBJECTIVE
To study the role of fibrinogen molecule in the pathogenesis of periodontal diseases.
METHODS
An in vitro cell culture model was used. Methyl-(3)H Thymidine radiolabeled Porphyromonas gingivalis (Pg) ATCC 33277 were examined for their ability to adhere to and invade the confluent monolayers of human oral epithelial KB cells with or without exogenous human fibrinogens by scintillation spectrometry.
RESULTS
The addition of exogenous fibrinogens made more amount of and higher ratios of adhesive and invasive Pg, in contrast to the group without exogenous fibrinogen (P < 0.001). At different concentrations of exogenous fibrinogen, the amount and ratios of adhesive and invasive Pg varied significantly (P < or = 0.007). The higher concentrations of exogenous fibrinogen was added, the greater amount and ratios of adhesive and invasive Pg were found.
CONCLUSIONS
Fibrinogen promotes the adherence of Pg to human oral epithelial cells and may play an important role in the pathogenesis of periodontal diseases.
目的
研究纤维蛋白原分子在牙周疾病发病机制中的作用。
方法
采用体外细胞培养模型。通过闪烁光谱法检测甲基 -（3）H 胸苷放射性标记的牙龈卟啉单胞菌（Pg）ATCC 33277 在有或无外源性人纤维蛋白原存在的情况下，黏附并侵入人口腔上皮 KB 细胞汇合单层的能力。
结果
与无外源性纤维蛋白原的组相比，添加外源性纤维蛋白原使黏附及侵入的 Pg 数量更多、比例更高（P < 0.001）。在不同浓度的外源性纤维蛋白原条件下，黏附及侵入的 Pg 数量和比例差异显著（P ≤ 0.007）。添加的外源性纤维蛋白原浓度越高，黏附及侵入的 Pg 数量和比例就越大。
结论
纤维蛋白原促进 Pg 对人口腔上皮细胞的黏附，可能在牙周疾病发病机制中起重要作用。
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