Umeda J E, Missailidis C, Longo P L, Anzai D, Wikström M, Mayer M P A
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Oral Microbiol Immunol. 2006 Dec;21(6):415-9. doi: 10.1111/j.1399-302X.2006.00312.x.
Adhesion to and invasion of epithelial cells by the periodontopathogen Porphyromonas gingivalis is promoted by the major fimbriae, encoded by fimA. The microorganism can be classified in six genotypes, based on fimA sequence, and genotype II strains are more prevalent than others in periodontitis patients. This study aimed to determine the adhesive and invasive abilities on KB cells of different fimA allelic variants of P. gingivalis isolates. Twenty-two isolates and six reference strains representing the six fimA genotypes and non-typeable strains were screened for their adhesion and invasion abilities on KB cells, using standard methods. All strains were able to adhere and, except for one, to invade KB cells. However, these properties were not homogeneous among strains belonging to the same genotype. There was no correlation between adhesion and invasion efficiencies. Isolate KdII 865 (fimA genotype II) was the most invasive and the second most adhesive strain, whereas reference strain ATCC 33277 (fimA I) showed a low adhesion ability but was highly invasive. These data indicated that fimA genotypes of P. gingivalis are not related to the adhesion and invasion abilities on KB cells, suggesting that the increased prevalence and proportion of certain genotypes may be attributed to other characteristics besides FimA variation.
由fimA编码的主要菌毛可促进牙周病原体牙龈卟啉单胞菌对上皮细胞的黏附和侵袭。根据fimA序列，该微生物可分为六种基因型，在牙周炎患者中，基因型II菌株比其他菌株更为普遍。本研究旨在确定牙龈卟啉单胞菌分离株不同fimA等位基因变体对KB细胞的黏附及侵袭能力。使用标准方法，对代表六种fimA基因型和不可分型菌株的22株分离株及6株参考菌株进行了对KB细胞的黏附及侵袭能力筛选。所有菌株均能黏附，除一株外，均能侵袭KB细胞。然而，在属于同一基因型的菌株中，这些特性并不一致。黏附效率与侵袭效率之间无相关性。分离株KdII 865（fimA基因型II）是侵袭性最强且黏附性第二强的菌株，而参考菌株ATCC 33277（fimA I）黏附能力低但侵袭性强。这些数据表明，牙龈卟啉单胞菌的fimA基因型与对KB细胞的黏附及侵袭能力无关，这表明某些基因型患病率和比例的增加可能归因于除FimA变异之外的其他特征。
