Yuan Jie, Zhu Lian, Wang Min, Cui Lei, Liu Wei, Cao Yi-lin
Department of Plastic and Reconstructive Surgery, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Feb;41(2):94-7.
To repair segmental mandibular defects with autogenous bone marrow stromal cells (BMSCs) and coralline hydroxyapatite.
Isolated BMSCs were in vitro expanded and osteogenically induced. In 11 canines, a 3 cm segmental mandibular defect in right mandible was created. Five canine's defects were repaired with cell-scaffold constructs made from induced BMSCs and coralline hydroxyapatite (CHA); Others were repaired with CHA as control. The engineered bone was evaluated by X-ray, CT, gross and histological examination, biomechanical test 12, 26, 32 weeks post-operation respectively.
BMSCs grew well on the CHA. X-ray and CT images showed better callus formation at connection sites in experimental group over time while worse formation at connection sites eventually in control group. At 32 weeks post-operation in experimental group, the defects were well repaired grossly. Histologically, there were bony healing and lamellar bone formation, in experimental group fibrous healing and woven bone formation in control group. Biomechanical test revealed no significant difference between experimental group and normal control group.
Canine segmental mandibular defects can be ultimately repaired with the tissue-engineered bone generated by autogenous osteogenic BMSCs and CHA scaffold.
用自体骨髓基质细胞（BMSCs）和珊瑚羟基磷灰石修复下颌骨节段性缺损。
分离培养BMSCs并进行体外扩增及成骨诱导。在11只犬的右侧下颌骨制造3cm节段性缺损。5只犬的缺损用诱导BMSCs与珊瑚羟基磷灰石（CHA）构建的细胞支架复合体修复；其余作为对照，用CHA修复。分别于术后12、26、32周通过X线、CT、大体及组织学检查、生物力学测试对工程化骨进行评估。
BMSCs在CHA上生长良好。随着时间推移，X线和CT图像显示实验组连接部位骨痂形成情况优于对照组，而对照组连接部位最终骨痂形成较差。术后32周，实验组缺损大体修复良好。组织学检查显示，实验组有骨愈合及板层骨形成，对照组为纤维愈合及编织骨形成。生物力学测试显示实验组与正常对照组无显著差异。
自体成骨BMSCs与CHA支架构建的组织工程骨可最终修复犬下颌骨节段性缺损。
