Vandenvelde C, Scheen R, Duys M, Corazza F, Van Beers D
Virology Department, Brugmann University Hospital, Brussels, Belgium.
Nouv Rev Fr Hematol (1978). 1991;33(4):293-7.
Available methods for the detection of minimal residual disease in T-cell malignancies are limited by their poor sensitivity and/or by their complexity. With the aim of avoiding these drawbacks, we used the Fast PCR technique in order to amplify V delta 1-(D delta 1)-(D delta 2)-J delta 1 and V gamma I family-J gamma junctional sequences from nucleated cells of boiled bone marrow. We were thus able to detect malignant T-cells down to a dilution of 1 in 665 nucleated marrow cells, in less than 4 hours after sampling. This new quantitative method is promising for monitoring therapy and detecting early disease relapse in T-lymphoproliferative disease, since it is 2 to 35 fold more sensitive than Southern blotting.
检测T细胞恶性肿瘤微小残留病的现有方法受到其低灵敏度和/或复杂性的限制。为了避免这些缺点，我们使用快速聚合酶链反应（Fast PCR）技术，从煮沸骨髓的有核细胞中扩增Vδ1-（Dδ1）-（Dδ2）-Jδ1和VγI家族-Jγ连接序列。因此，我们能够在取样后不到4小时内，检测到低至每665个有核骨髓细胞中1个稀释度的恶性T细胞。这种新的定量方法有望用于监测T淋巴细胞增殖性疾病的治疗和检测早期疾病复发，因为它的灵敏度比Southern印迹法高2至35倍。
