从同一批次的鱼腥藻Anabaena PCC 7119中纯化铁氧化还原蛋白-NADP⁺还原酶、黄素氧还蛋白和铁氧化还原蛋白。
Purification of ferredoxin-NADP+ reductase, flavodoxin and ferredoxin from a single batch of the cyanobacterium Anabaena PCC 7119.
作者信息
Pueyo J J, Gómez-Moreno C
机构信息
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Spain.
出版信息
Prep Biochem. 1991;21(4):191-204. doi: 10.1080/10826069108018571.
Methods are described for the simultaneous isolation of ferredoxin-NADP+ reductase, ferredoxin and flavodoxin from large quantities of the cyanobacterium Anabaena PCC 7119 allowing the use of a single batch of cells. The ultraviolet-visible spectra and the extinction coefficients of ferredoxin-NADP+ reductase and ferredoxin were determined. The purification procedure also yields enriched fractions of phycobiliproteins and cytochrome c553.
本文描述了从大量鱼腥藻Anabaena PCC 7119中同时分离铁氧化还原蛋白-NADP⁺还原酶、铁氧化还原蛋白和黄素氧还蛋白的方法，从而可以使用同一批细胞。测定了铁氧化还原蛋白-NADP⁺还原酶和铁氧化还原蛋白的紫外可见光谱及消光系数。该纯化程序还能得到富含藻胆蛋白和细胞色素c553的组分。
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