Teshfam M, Brujeni Gh Nikbakht, Hassanpour H
Department of Physiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Br Poult Sci. 2006 Apr;47(2):223-9. doi: 10.1080/00071660600611169.
To clarify the effect of cold-induced pulmonary hypertension on endothelial and inducible nitric oxide synthase (eNOS and iNOS) mRNA expression in the lung of broiler chickens, semi quantitative reverse transcription-PCR was performed on total RNAs isolated from lungs of the broiler chickens exposed to 5 weeks of cold stress. The eNOS gene was expressed increasingly with the increasing age during the rearing period. Comparing the treatment group with its related control group eNOS was expressed significantly only at d 21. Expression of iNOS mRNA also increased in both control and treatment groups with increasing age until d 28 and then decreased at d 35 and 42. Comparing the treatment group with its control group, iNOS mRNA level was significantly higher at 21 d of age in the cold-exposed chickens. It was concluded that, although cold exposure could significantly increase eNOS and iNOS gene expression, cold-induced pulmonary hypertension is not associated with significant variations of eNOS and iNOS expression in the lungs of broiler chickens.
为阐明冷应激诱导的肺动脉高压对肉鸡肺组织中内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）mRNA表达的影响，对暴露于5周冷应激的肉鸡肺组织分离的总RNA进行半定量逆转录-聚合酶链反应。在饲养期内，eNOS基因表达随年龄增长而增加。将处理组与其相关对照组比较，eNOS仅在第21天有显著表达。iNOS mRNA表达在对照组和处理组中均随年龄增长而增加，直至第28天，然后在第35天和第42天下降。将处理组与其对照组比较，冷暴露鸡在21日龄时iNOS mRNA水平显著更高。得出的结论是，虽然冷暴露可显著增加eNOS和iNOS基因表达，但冷应激诱导的肺动脉高压与肉鸡肺组织中eNOS和iNOS表达的显著变化无关。
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