Wan Li Li, Xia Jiahong, Ye Duoyun, Liu Jinping, Chen Jun, Wang Gang
Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Cardiovasc Ther. 2009 Spring;27(1):28-33. doi: 10.1111/j.1755-5922.2009.00071.x.
Previous studies have suggested that reactive oxygen species (ROS), endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) are involved in the pathophysiology of myocardial ischemia-reperfusion injury (MIRI). The NOX family of NADPH oxidases share the capacity to generate superoxide and ROS. Several studies have demonstrated that quercetin possesses a protective effect against MIRI. Our aim is to investigate the effects of quercetin on NOX2, eNOS, and iNOS after MIRI in rabbits. New Zealand rabbits were subjected to 30 min of myocardial ischemia followed by 12 h of reperfusion. They were then randomly assigned to four experimental groups: control, I/R (ischemia/reperfusion), quercetin (Que), I/R + Que. Gene and protein expression of NOX2, eNOS, and iNOS were compared. Both in real-time PCR and in the Western blotting studies, myocardial ischemia-reperfusion-induced NOX2 and iNOS expression were enhanced (P < 0.01) but eNOS mRNA and protein expression in I/R hearts were not significantly different from those in control (P < 0.01). Administration of quercetin reduced NOX2, eNOS, and iNOS mRNA and protein expression both in control and in I/R heart (P < 0.01). Gene and protein expression of NOX2 and iNOS were increased after MIRI. Quercetin not only inhibited myocardial ischemia-reperfusion-induced NOX2 and iNOS mRNA and protein expression but also inhibited eNOS mRNA and protein expression.
先前的研究表明，活性氧（ROS）、内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）参与了心肌缺血再灌注损伤（MIRI）的病理生理过程。NADPH氧化酶的NOX家族具有产生超氧化物和ROS的能力。多项研究表明，槲皮素对MIRI具有保护作用。我们的目的是研究槲皮素对兔MIRI后NOX2、eNOS和iNOS的影响。将新西兰兔进行30分钟的心肌缺血，随后再灌注12小时。然后将它们随机分为四个实验组：对照组、I/R（缺血/再灌注）组、槲皮素（Que）组、I/R + Que组。比较NOX2、eNOS和iNOS的基因和蛋白表达。在实时PCR和蛋白质印迹研究中，心肌缺血再灌注诱导的NOX2和iNOS表达均增强（P < 0.01），但I/R心脏中的eNOS mRNA和蛋白表达与对照组相比无显著差异（P < 0.01）。给予槲皮素可降低对照组和I/R心脏中NOX2、eNOS和iNOS的mRNA和蛋白表达（P < 0.01）。MIRI后NOX2和iNOS的基因和蛋白表达增加。槲皮素不仅抑制心肌缺血再灌注诱导的NOX2和iNOS的mRNA和蛋白表达，还抑制eNOS的mRNA和蛋白表达。
