Tan X, Sun W-D, Li J-Ch, Pan J-Q, Wang X-L
Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P.R. China.
Br Poult Sci. 2006 Apr;47(2):230-6. doi: 10.1080/00071660600611227.
The present study was conducted to examine the effect of supplemental L-arginine on pulmonary arteriole protein kinase Calpha (PKCalpha) expression in broilers exposed to cool temperature, to investigate further the molecular mechanisms of supplemental L-arginine on modulating pulmonary vascular functions in hypertensive broilers. Broilers were subjected to sub-thermoneutral (cool) temperature to induce pulmonary hypertension syndrome (PHS), and an additional 10 g/kg L-arginine was added to the basal diet to evaluate the effects of supplemental L-arginine on PHS mortality, plasma nitric oxide (NO) production and pulmonary arterioles PKCalpha expression. Supplemental L-arginine reduced PHS mortality but did not affect right/total ventricle (RV/TV) ratios in clinically healthy birds. Birds fed additional L-arginine had increased plasma NO and decreased PKCalpha protein expression in pulmonary arterioles; NO production was negatively correlated with PKCalpha expression. These results demonstrated that supplemental L-arginine diminished PKCalpha expression in birds exposed to cool temperature. It is suggested that NO-induced loss of PKCalpha expression might be partially responsible for its effects on dilating pulmonary vasculature and inhibiting pulmonary vascular remodelling in vivo.
本研究旨在探讨补充L-精氨酸对处于低温环境下的肉鸡肺小动脉蛋白激酶Cα（PKCα）表达的影响，以进一步研究补充L-精氨酸调节高血压肉鸡肺血管功能的分子机制。将肉鸡置于亚热中性（低温）环境中以诱发肺动脉高压综合征（PHS），并在基础日粮中额外添加10 g/kg L-精氨酸，以评估补充L-精氨酸对PHS死亡率、血浆一氧化氮（NO）生成以及肺小动脉PKCα表达的影响。补充L-精氨酸降低了PHS死亡率，但对临床健康鸡的右心室/总心室（RV/TV）比值没有影响。额外添加L-精氨酸的鸡血浆NO增加，肺小动脉中PKCα蛋白表达降低；NO生成与PKCα表达呈负相关。这些结果表明，补充L-精氨酸可减少处于低温环境下鸡的PKCα表达。提示NO诱导的PKCα表达缺失可能部分解释了其在体内扩张肺血管和抑制肺血管重塑的作用机制。
