Schettini G, Grimaldi M, Landolfi E, Meucci O, Ventra C, Florio T, Scorziello A, Marino A
Dept. of Communicative Science, 2. School of Medicine, University of Naples.
Acta Neurol (Napoli). 1991 Aug;13(4):361-7.
Interleukin-6 (IL-6) is a pleiotropic cytokine exerting many immunological and non immunological actions. The cytokine binds to a specific receptor, whose activation induces the association with a novel transducer, the glycoprotein gp 130. Here we present our results about the effect of IL-6 on both hormone secretion and second messenger systems at pituitary level, and the production of IL-6 from cells of central nervous system. IL-6 inhibited basal, VIP and TRH-stimulated prolactin (PRL) secretion from single lactotropes, studied by means of reverse hemolytic plaque assay, whereas in primary cultures of anterior pituitary cells, according to the literature, the cytokine stimulated prolactin secretion. IL-6 did not affect basal adenylate cyclase activity, inositol phosphate production, and cytosolic calcium concentration. Conversely, the preincubation of pituitary cells with interleukin-6 for 20 min significantly reduced VIP- and forskolin-stimulated adenylate cyclase activity, as well as inositol phosphate production and free cytosolic calcium increase induced by TRH.
白细胞介素-6（IL-6）是一种多效性细胞因子，具有多种免疫和非免疫作用。该细胞因子与一种特异性受体结合，其激活会诱导与一种新型转导分子——糖蛋白gp130结合。在此，我们展示了关于IL-6对垂体水平激素分泌和第二信使系统的影响，以及中枢神经系统细胞产生IL-6的研究结果。通过反向溶血空斑试验研究发现，IL-6抑制单个催乳素细胞基础、血管活性肠肽（VIP）和促甲状腺激素释放激素（TRH）刺激的催乳素（PRL）分泌，而根据文献报道，在垂体前叶细胞原代培养中，该细胞因子会刺激催乳素分泌。IL-6不影响基础腺苷酸环化酶活性、肌醇磷酸生成和胞质钙浓度。相反，将垂体细胞与白细胞介素-6预孵育20分钟可显著降低VIP和福斯高林刺激的腺苷酸环化酶活性，以及TRH诱导的肌醇磷酸生成和游离胞质钙增加。
