Wang Huan-qin, Liu Wei-bing, Yang Dong-rong, Liang Yong, Wang Jun-wei, Zhang Lian-shan, Liu Jia-wei, Tao San-ju, Lv Xin-jun, Liang Guo-dong
Institute of Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2006 Mar;20(1):52-5.
To study the arboviruses carried by mosquitoes collected in Hebei Province.
Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR.
Totally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus.
Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.
研究河北省采集的蚊子所携带的虫媒病毒。
从蚊子活动场所采集样本并储存于液氮中备用。将20至30只蚊子组成的样本池进行灭菌后研磨，离心后的上清液接种到C6/36细胞上，连续传代3次观察细胞病变效应。通过免疫荧光分析（IFA）和逆转录聚合酶链反应（RT-PCR）鉴定阳性分离株。
从河北省涉县两个村庄共采集1310只蚊子，分为46个样本池并分别研磨。获得13个阳性分离株。其中两个分离株与甲病毒抗体发生反应，并被甲病毒引物扩增，核苷酸序列与盖塔病毒（AY702913.1）的同源性最高（98%），因此这两个分离株被鉴定为盖塔病毒。
从河北省的蚊子中分离出盖塔病毒。这是中国内陆首次分离出盖塔病毒的报道。
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