[2007年山西省部分地区虫媒病毒调查]

[Arbovirus investigation in some regions of Shanxi province in 2007].

作者信息

Li Ming-Hua, Meng Wei-Shan, Fu Shi-Hong, Cheng Jing-Xia, Zhao Jun-Ying, Kong Xiang-Sheng, Dai Pei-Fang, Liang Guo-Dong

机构信息

State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Feb;23(1):32-4.

PMID:19799012

Abstract

OBJECTIVE

To investigate arboviruses in some regions of Shanxi province, isolation and identification for arbovirus activity from mosquitoes was conducted.

METHODS

Mosquitoes were collected from these area in 2007 and then used for virus isolation by cell culture. The virus isolates were identified by molecular biology and the sequences were analyzed by bioinformatics.

RESULTS

Ten Banna virus (strains SX0765, SX0766, SX0767, SX0771, SX0789, SX0790, SX0793, SX0794, SX0795, SX0796) were isolated, and two Liaoning virus were also isolated from isolates SX0771, SX0794. Phylogenetic tree of the Banna virus isolates showed that ten strains are located in a distinct branch from all of the other Chinese Banna virus isolates. The homology is between 89.7% and 94.1%.

CONCLUSION

Ten Banna virus and two Liaoning virus were isolated during this arbovirus investigation in Shanxi province. New Banna virus isolates showed a distinct phylogenetic relationship with the other Chinese Banna virus strains.

摘要

目的

调查山西省部分地区虫媒病毒，对蚊虫进行虫媒病毒活性的分离与鉴定。

方法

于2007年从这些地区采集蚊虫，然后通过细胞培养进行病毒分离。采用分子生物学方法对病毒分离株进行鉴定，并运用生物信息学方法对序列进行分析。

结果

分离出10株版纳病毒（毒株SX0765、SX0766、SX0767、SX0771、SX0789、SX0790、SX0793、SX0794、SX0795、SX0796），同时从毒株SX0771、SX0794中分离出2株辽宁病毒。版纳病毒分离株的系统发育树显示，这10个毒株位于与所有其他中国版纳病毒分离株不同的分支上。同源性在89.7%至94.1%之间。