Pirri Giovanna, Chiari Marcella, Damin Francesco, Meo Alessandra
Istituto di Chimica del Riconoscimento Molecolare, CNR, Milano, Italy.
Anal Chem. 2006 May 1;78(9):3118-24. doi: 10.1021/ac0521091.
The reversible addition-fragmentation chain-transfer polymerization was used to prepare microarray slides grafted with polymer brushes for DNA-based applications. Block copolymer brushes of N,N-dimethylacrylamide (DMA) and glycidyl methacrylate (GMA), poly(DMA-b-GMA) were prepared by extending living poly(dimethylacrylamide) chains. The functional surface was used as a substrate for oligonucleotide hybridization experiments. The results were compared to those provided by glass slides coated by a self-assembled monolayer made of (3-glycidyloxypropyl)trimethoxysilane. Surfaces coated with block polymer brushes bearing oxirane groups are more efficient as substrates for oligonucleotide hybridization than surfaces coated with nonpolymeric self-assembled monolayers containing the same functional group. The high probe grafting density and hybridization efficiency achieved with this polymeric coating reveal the importance of the block architecture to ensure good accessibility of the immobilized probe. The new surface was characterized by static angle measurements and diffuse reflectance FT-IR spectroscopy on a silica model system.
采用可逆加成-断裂链转移聚合反应制备了接枝有聚合物刷的微阵列载玻片，用于基于DNA的应用。通过延长活性聚（N,N-二甲基丙烯酰胺）链制备了N,N-二甲基丙烯酰胺（DMA）和甲基丙烯酸缩水甘油酯（GMA）的嵌段共聚物刷，即聚（DMA-b-GMA）。将该功能化表面用作寡核苷酸杂交实验的底物。将结果与由（3-缩水甘油氧基丙基）三甲氧基硅烷制成的自组装单分子层涂覆的载玻片所提供的结果进行了比较。带有环氧乙烷基团的嵌段聚合物刷涂覆的表面作为寡核苷酸杂交的底物比含有相同官能团的非聚合物自组装单分子层涂覆的表面更有效。通过这种聚合物涂层实现的高探针接枝密度和杂交效率揭示了嵌段结构对于确保固定探针良好可及性的重要性。在二氧化硅模型系统上通过静态接触角测量和漫反射傅里叶变换红外光谱对新表面进行了表征。
