虹鳟鱼（Oncorhynchus mykiss）肝脏酶合成抗坏血酸-2-磷酸酯。

Synthesis of ascorbyl-2-phosphate by liver enzyme of rainbow trout Oncorhynchus mykiss.

Miyasaki T, Sato M, Yoshinaka R, Sakaguchi M

Department of Fisheries, Faculty of Agriculture, Kyoto University, Japan.

Comp Biochem Physiol B. 1991;100(4):711-6. doi: 10.1016/0305-0491(91)90278-l.

Abstract

Ascorbyl-2-monophosphate was enzymatically formed in the reaction mixture of L-ascorbic acid, pyrophosphate and the homogenate of rainbow trout Oncorhynchus mykiss liver. 2. The liver had the highest activity among the liver, spleen, kidney, stomach, pyloric caeca and intestine. 3. Pyrophosphate, triphosphate, ADP and ATP were good substrates as phosphoryl donors, but phosphoric acid and AMP were poor. 4. The optimum pH and temperature of AP-forming activity in the liver were around 5.0 and 30 degrees C, respectively. 5. The Km values for ascorbic acid and pyrophosphate were 370 and 83 mM, respectively.

摘要

在L-抗坏血酸、焦磷酸和虹鳟鱼（Oncorhynchus mykiss）肝脏匀浆的反应混合物中酶促形成了抗坏血酸-2-单磷酸酯。2. 肝脏在肝脏、脾脏、肾脏、胃、幽门盲囊和肠道中活性最高。3. 焦磷酸、三磷酸、ADP和ATP作为磷酰基供体是良好的底物，但磷酸和AMP则较差。4. 肝脏中形成AP活性的最适pH和温度分别约为5.0和30℃。5. 抗坏血酸和焦磷酸的Km值分别为370 mM和83 mM。