Huang Zhi Gang, Ran Zhi Hua, Lu Wei, Xiao Shu Dong
Shanghai Institute of Digestive Diseases, Renji Hospital, Shanghai Second Medical University, Shanghai, China.
Chin J Dig Dis. 2006;7(2):97-102. doi: 10.1111/j.1443-9573.2006.00254.x.
To investigate the changes in the gene expression profile in colon cancer to further identify gene markers that may be useful in the management of this disease.
Data from serial analysis of gene expression (SAGE) collected by the Cancer Genome Anatomy Project (CGAP) were used to detect the difference in gene expression between normal tissue and colon cancer, and were further confirmed in a sample of 20 patients using RT-PCR. To identify the functions of differential genes in regulating the cell growth of colon cancer, RNA interference (RNAi) was used to block one of these genes in the colon cancer cell line HCT-116.
Expression changes of greater than twofold in two SAGE libraries of colon cancer compared to two of normal tissue were observed for 216 tags of a total of 195,160 transcript tags (54 up-regulated genes and 136 down-regulated genes). Subsequent analysis of 17 genes by RT-PCR confirmed the reliability of this analysis. RNAi-mediated blockage of one of these genes, transforming growth factor (TGF)beta1, significantly reduced the growth of a colon cancer cell line.
The combination of CGAP analysis and RNAi provides an excellent system to rapidly define the specific genes that are up-regulated in cancer to impact the growth of cancer cells. Further study on these differential overexpressed genes may provide gene markers for the detection and treatment of colon cancer.
研究结肠癌基因表达谱的变化，以进一步确定可能有助于该疾病管理的基因标志物。
利用癌症基因组解剖计划（CGAP）收集的基因表达系列分析（SAGE）数据，检测正常组织与结肠癌之间的基因表达差异，并在20例患者的样本中使用逆转录聚合酶链反应（RT-PCR）进一步验证。为了确定差异基因在调节结肠癌细胞生长中的功能，使用RNA干扰（RNAi）在结肠癌细胞系HCT-116中阻断其中一个基因。
在总共195,160个转录本标签中的216个标签中，观察到与两个正常组织的SAGE文库相比，结肠癌的两个SAGE文库中的表达变化超过两倍（54个上调基因和136个下调基因）。随后通过RT-PCR对17个基因进行分析，证实了该分析的可靠性。RNAi介导的对其中一个基因转化生长因子（TGF）β1的阻断，显著降低了结肠癌细胞系的生长。
CGAP分析和RNAi的结合提供了一个出色的系统，可快速确定癌症中上调的特定基因，从而影响癌细胞的生长。对这些差异过表达基因的进一步研究可能为结肠癌的检测和治疗提供基因标志物。
