Christ George J, Hsieh Yi, Zhao Weixin, Schenk Gregory, Venkateswarlu Karicheti, Wang Hong-Zhan, Tar Moses T, Melman Arnold
Department of Regenerative Medicine, Wake Forest University Baptist Medical Center, Winston-Salem, NC 27157, USA.
BJU Int. 2006 May;97(5):1076-82. doi: 10.1111/j.1464-410X.2006.06058.x.
To establish the methods, feasibility and utility of evaluating the impact of diabetes on bladder and erectile function in the same rat, as more than half of diabetic patients have bladder dysfunction, and half of diabetic men have erectile dysfunction, but the severity of coincident disease has not been rigorously assessed.
In all, 16 F-344 rats had diabetes induced by streptozotocin (STZ), and were divided into insulin-treated (five) and untreated (11), and compared with age-matched controls (10), all assessed in parallel. All STZ rats were diabetic for 8-11 weeks. Cystometric studies were conducted on all rats, with cavernosometric studies conducted on a subset of rats.
There were insulin-reversible increases in the following cystometric variables; bladder weight, bladder capacity, micturition volume, residual volume, micturition pressure and spontaneous activity (P < 0.05, in all, one-way analysis of variance, anova). Cavernosometry showed a diabetes-related, insulin-reversible decline in the cavernosal nerve-stimulated intracavernosal pressure (ICP) response at all levels of current stimulation (P < 0.05, in all one-way anova). Plotting erectile capacity (i.e. ICP) against bladder capacity showed no correlation between the extent of the decline in erectile capacity and the magnitude of the increase in bladder capacity.
These studies extend previous work to indicate that the extent of diabetes-related bladder and erectile dysfunction can vary in the same rat. As such, these findings highlight the importance of evaluating the impact of diabetes on multiple organ systems in the lower urinary tract. Future studies using this model system should lead to a better understanding of the initiation, development, progression and coincidence of these common diabetic complications.
建立在同一只大鼠中评估糖尿病对膀胱和勃起功能影响的方法、可行性及实用性。因为超过半数的糖尿病患者存在膀胱功能障碍，且半数糖尿病男性有勃起功能障碍，但合并疾病的严重程度尚未得到严格评估。
总共16只F-344大鼠通过链脲佐菌素（STZ）诱导患糖尿病，分为胰岛素治疗组（5只）和未治疗组（11只），并与年龄匹配的对照组（10只）进行平行评估。所有STZ大鼠患糖尿病8至11周。对所有大鼠进行膀胱测压研究，对部分大鼠进行海绵体测压研究。
以下膀胱测压变量出现胰岛素可逆性增加；膀胱重量、膀胱容量、排尿量、残余尿量、排尿压力和自发活动（均P<0.05，单因素方差分析，anova）。海绵体测压显示，在所有电流刺激水平下，糖尿病相关的、胰岛素可逆的海绵体神经刺激引起的海绵体内压（ICP）反应下降（均P<0.05，单因素anova）。绘制勃起能力（即ICP）与膀胱容量的关系图显示，勃起能力下降程度与膀胱容量增加幅度之间无相关性。
这些研究扩展了先前的工作，表明在同一只大鼠中，糖尿病相关的膀胱和勃起功能障碍程度可能不同。因此，这些发现突出了评估糖尿病对下尿路多个器官系统影响的重要性。使用该模型系统的未来研究应有助于更好地理解这些常见糖尿病并发症的发生、发展、进展和合并情况。
