Kovács K J, Péczely P
Institute of Experimental Medicine, Budapest, Hungary.
Gen Comp Endocrinol. 1991 Nov;84(2):192-8. doi: 10.1016/0016-6480(91)90042-5.
A direct radioimmunoassay for the determination of avian adrenocorticotropin (ACTH) in a small volume of plasma was developed using an antiserum specific for N-terminal region of the ACTH molecule. The sensitivity of the two stage assay is 0.1 fmol ACTH/tube. The specificity of the antiserum was tested by its cross reactions with synthetic ACTH fragments and by comparing curves obtained by dilution of different plasma specimens to that of ACTH reference standard. Adrenocorticotropin responses of chronically cannulated geese to ether stress were evaluated and compared to changes of plasma corticosterone (B) concentration over a 2-hr period. ACTH showed a maximum between 5 and 10 min after ether exposure, while B peak appeared 10-15 min later. Thirty minutes after ether inhalation plasma ACTH returned to the baseline, while B response was longer-lasting and decreased to the resting level between 60 and 120 min. Basal and stress-induced ACTH plasma levels were also investigated in male and female gonadectomized and thyroidectomized geese. Castration increased, while thyroidectomy decreased the basal ACTH concentration. These endocrine manipulations did not, however, markedly affect the stress-induced ACTH hypersecretion except in thyroidectomized ganders, where the increment of plasma ACTH 10 min after ether inhalation was significantly lower than in sham operated control geese.
利用针对促肾上腺皮质激素（ACTH）分子N端区域的特异性抗血清，开发了一种用于测定少量血浆中禽促肾上腺皮质激素（ACTH）的直接放射免疫测定法。两阶段测定法的灵敏度为0.1 fmol ACTH/管。通过抗血清与合成ACTH片段的交叉反应以及将不同血浆标本稀释后得到的曲线与ACTH参考标准曲线进行比较，来测试抗血清的特异性。评估了长期插管鹅对乙醚应激的促肾上腺皮质激素反应，并与2小时内血浆皮质酮（B）浓度的变化进行了比较。ACTH在乙醚暴露后5至10分钟出现最大值，而B峰值在10至15分钟后出现。乙醚吸入30分钟后，血浆ACTH恢复到基线水平，而B反应持续时间更长，并在60至120分钟之间降至静息水平。还研究了雄性和雌性去性腺和甲状腺切除的鹅的基础和应激诱导的血浆ACTH水平。去势会增加基础ACTH浓度，而甲状腺切除会降低基础ACTH浓度。然而，这些内分泌操作并没有显著影响应激诱导的ACTH分泌过多，除了甲状腺切除的雄鹅，其在乙醚吸入10分钟后血浆ACTH的增量明显低于假手术对照鹅。
