Terano Y, Tomii A, Masugi F
Department of Physiology, Osaka City University Medical School.
Jpn J Med Sci Biol. 1991 Jun;44(3):123-39. doi: 10.7883/yoken1952.44.123.
Two monoclonal antibodies (249F8 and 278A9) to ouabain were produced by somatic cell fusion. They reacted in dose-dependent manners with ouabain and digoxin. These antibodies were supposed to recognize the partial structure important for cardiac compounds to show their pharmacological activity, Na+, K(+)-ATPase inhibition in a competitive mode against K+. These monoclonal antibodies may be useful for the immunochemical isolation, the structural elucidation, and the quantitative measurement of putative endogenous digoxin-like factors.
通过体细胞融合制备了两种针对哇巴因的单克隆抗体（249F8和278A9）。它们与哇巴因和地高辛呈剂量依赖性反应。这些抗体被认为能够识别对心脏化合物显示其药理活性（即对钾离子呈竞争性抑制模式的钠钾ATP酶）至关重要的部分结构。这些单克隆抗体可能有助于免疫化学分离、结构解析以及对假定的内源性地高辛样因子进行定量测定。
