冈比亚按蚊的自然疟疾感染受单个基因组控制区域调控。

Natural malaria infection in Anopheles gambiae is regulated by a single genomic control region.

作者信息

Riehle Michelle M, Markianos Kyriacos, Niaré Oumou, Xu Jiannong, Li Jun, Touré Abdoulaye M, Podiougou Belco, Oduol Frederick, Diawara Sory, Diallo Mouctar, Coulibaly Boubacar, Ouatara Ahmed, Kruglyak Leonid, Traoré Sékou F, Vernick Kenneth D

机构信息

Center for Microbial and Plant Genomics and Department of Microbiology, University of Minnesota, St. Paul, MN 55108, USA.

出版信息

Science. 2006 Apr 28;312(5773):577-9. doi: 10.1126/science.1124153.

DOI:10.1126/science.1124153

PMID:16645095

Abstract

We surveyed an Anopheles gambiae population in a West African malaria transmission zone for naturally occurring genetic loci that control mosquito infection with the human malaria parasite, Plasmodium falciparum. The strongest Plasmodium resistance loci cluster in a small region of chromosome 2L and each locus explains at least 89% of parasite-free mosquitoes in independent pedigrees. Together, the clustered loci form a genomic Plasmodium-resistance island that explains most of the genetic variation for malaria parasite infection of mosquitoes in nature. Among the candidate genes in this chromosome region, RNA interference knockdown assays confirm a role in Plasmodium resistance for Anopheles Plasmodium-responsive leucine-rich repeat 1 (APL1), encoding a leucine-rich repeat protein that is similar to molecules involved in natural pathogen resistance mechanisms in plants and mammals.

摘要

我们在西非疟疾传播区对冈比亚按蚊种群进行了调查，以寻找控制蚊子感染人类疟原虫——恶性疟原虫的自然发生的基因位点。最强的疟原虫抗性位点聚集在2L染色体的一个小区域，每个位点在独立谱系中解释了至少89%无寄生虫的蚊子。这些聚集的位点共同形成了一个基因组疟原虫抗性岛，解释了自然界中蚊子感染疟原虫的大部分遗传变异。在这个染色体区域的候选基因中，RNA干扰敲除试验证实了按蚊疟原虫反应性富含亮氨酸重复序列1（APL1）在疟原虫抗性中的作用，APL1编码一种富含亮氨酸重复序列的蛋白质，类似于植物和哺乳动物中参与天然病原体抗性机制的分子。