Bilous A M, McKay M, Milliken J
Department of Anatomical Pathology, Westmead Hospital, N.S.W., Australia.
Pathology. 1991 Oct;23(4):282-5. doi: 10.3109/00313029109063589.
Methods of assessing tumor proliferation rates include mitosis counting, flow cytometry and thymidine labelling. While the former is inaccurate and poorly reproducible, the latter methods are time consuming and expensive to perform. Ki-67 is a monoclonal mouse antibody which has been shown to react with a nuclear antigen in proliferating cells. Frozen sections from 75 specimens of breast carcinoma were immunostained with this antibody using an immunoperoxidase technique. The percentage of tumor cells stained, the Ki-67 score, was then compared with a number of pathological and clinical variables in the patients concerned. A positive correlation was seen between the Ki-67 score and mitotic rate (r = 0.71); and a negative correlation was seen between Ki-67 score and estrogen receptor status (r = -0.4). Ki-67 immunostaining may represent a cheap and reproducible method of assessing proliferation rates of breast carcinomas which is applicable in routine laboratories. Further prospective studies are being undertaken to assess its contribution to prognosis.
评估肿瘤增殖率的方法包括有丝分裂计数、流式细胞术和胸腺嘧啶核苷标记法。虽然前者不准确且重复性差，但后两种方法实施起来既耗时又昂贵。Ki-67是一种单克隆小鼠抗体，已证明它能与增殖细胞中的一种核抗原发生反应。采用免疫过氧化物酶技术，用该抗体对75例乳腺癌标本的冰冻切片进行免疫染色。然后将染色的肿瘤细胞百分比即Ki-67评分，与相关患者的一些病理和临床变量进行比较。发现Ki-67评分与有丝分裂率之间呈正相关（r = 0.71）；Ki-67评分与雌激素受体状态之间呈负相关（r = -0.4）。Ki-67免疫染色可能是一种廉价且可重复的评估乳腺癌增殖率的方法，适用于常规实验室。正在进行进一步的前瞻性研究以评估其对预后的作用。
