Andersson S, Hellström A-C, Ren Zhi-Ping, Wilander E
Department of Obstetrics and Gynecology, Institute for Clinical Science, Karolinska University Hospital, Huddinge, Karolinska Institute, 14186 Stockholm, Sweden.
Med Oncol. 2006;23(1):113-9. doi: 10.1385/MO:23:1:113.
Thirty tumors were collected from our archive of cervical adenocarcinomas. They were examined with respect to the content of oncogenic HPV and presence of mutations in the p53 gene exons 5 through 8. Furthermore, available clinical information on the cases was reviewed. For the detection of p53 gene and presence of oncogenic HPV, PCR followed by direct sequence analysis of the amplified DNA was employed. Seventeen tumors were identified as HPV-positive, comprising both HPV types 18 and 16. Six cases showed a p53 gene mutation, of which five were of the missence and one of the silent type. No statistical correlation between the occurrence of oncogenic HPV and presence of p53 gene mutation (p = 0.67) was recorded. Among the tumors with p53 gene mutation, three were HPV-positive and three were HPV-negative. The determination of p53 gene mutations was not related to clinical findings such as the stage of the tumor or presence of metastases of the lymph nodes. However, p53 gene mutations were somewhat more prevalent in low differentiated tumors (p < 0.02). The results indicate that oncogenic HPV and p53 gene mutations have independent carcinogenic roles in cervical adenocarcinomas.
从我们的宫颈腺癌存档中收集了30个肿瘤样本。对其致癌性人乳头瘤病毒（HPV）含量以及p53基因第5至8外显子的突变情况进行了检测。此外，还回顾了这些病例的现有临床信息。为检测p53基因及致癌性HPV的存在情况，采用聚合酶链反应（PCR），随后对扩增的DNA进行直接序列分析。17个肿瘤被鉴定为HPV阳性，包括HPV 18型和16型。6例显示p53基因突变，其中5例为错义突变，1例为沉默突变。未发现致癌性HPV的存在与p53基因突变之间存在统计学相关性（p = 0.67）。在p53基因突变的肿瘤中，3例为HPV阳性，3例为HPV阴性。p53基因突变的测定与肿瘤分期或淋巴结转移等临床发现无关。然而，p53基因突变在低分化肿瘤中更为普遍（p < 0.02）。结果表明，致癌性HPV和p53基因突变在宫颈腺癌中具有独立的致癌作用。
