Chao Bing-Fong, Chen Chao-Jung, Li Fu-An, Her Guor-Rong
Department of Chemistry, National Taiwan University, Taipei.
Electrophoresis. 2006 Jun;27(11):2083-90. doi: 10.1002/elps.200500811.
A sheathless interface has been developed for coupling CE with electrospray IT mass spectrometer. This interface utilized a pulsed ESI source. The use of a pulsed electrospray source allows the use of a sprayer with larger orifice, and thus alleviates the problem of column clogging during conductive coating and CE analysis. A pulsed ESI source operated at 20 Hz and 20% duty cycle was found to produce the optimal signals. For better signals, the maximum ion injection time in the IT mass spectrometer has to be set to a value close to the actual spraying time (10 ms). Using a sprayer with 50 microm od, more stable and enhanced signals were obtained in comparison with continuous CE-ESI-MS under the same flow rate (150 nL/min). The utility of this design is demonstrated with the analysis of synthetic drugs by CE-MS.
已开发出一种用于将毛细管电泳（CE）与电喷雾离子阱质谱仪耦合的无鞘接口。该接口采用了脉冲电喷雾离子源。使用脉冲电喷雾源允许使用具有较大孔径的喷雾器，从而缓解了在导电涂层和CE分析过程中柱堵塞的问题。发现以20 Hz和20%占空比运行的脉冲电喷雾离子源可产生最佳信号。为了获得更好的信号，离子阱质谱仪中的最大离子注入时间必须设置为接近实际喷雾时间（10 ms）的值。在相同流速（150 nL/min）下，与连续CE-ESI-MS相比，使用外径为50微米的喷雾器可获得更稳定且增强的信号。通过CE-MS对合成药物的分析证明了该设计的实用性。
