Huang Yifan, Hsu Jason C, Peruggia Mario, Scott Abigail A
H. Lee Moffitt Cancer Center and Research Institute, USA.
Stat Appl Genet Mol Biol. 2006;5:Article4. doi: 10.2202/1544-6115.1122. Epub 2006 Feb 24.
Maintenance genes can be used for normalization in the comparison of gene expressions. Even though the absolute expression levels of maintenance genes may vary considerably among different tissues or cells, a set of maintenance genes may provide suitable normalization if their expression levels are relatively constant in the specific tissues or cells of interest. A statistical procedure is proposed to select maintenance genes for normalization of gene expression data from tissues or cells of interest. This procedure is based on simultaneous confidence intervals for practical equivalence of relative gene expressions in these tissues or cells. As an illustration, the procedure is applied to the maintenance gene expression data from Vandesompele et al. (2002).
维持基因可用于基因表达比较中的标准化。尽管维持基因的绝对表达水平在不同组织或细胞间可能有很大差异，但如果其在感兴趣的特定组织或细胞中的表达水平相对恒定，一组维持基因可能会提供合适的标准化。本文提出了一种统计方法来选择维持基因，用于对来自感兴趣组织或细胞的基因表达数据进行标准化。该方法基于这些组织或细胞中相对基因表达实际等效性的同时置信区间。作为示例，该方法应用于Vandesompele等人（2002年）的维持基因表达数据。
