硫氧还蛋白-1在琥珀酸生育酚和肿瘤坏死因子相关凋亡诱导配体诱导间皮瘤细胞凋亡中的作用
Role of thioredoxin-1 in apoptosis induction by alpha-tocopheryl succinate and TNF-related apoptosis-inducing ligand in mesothelioma cells.
作者信息
Freeman Ruth E, Neuzil Jiri
机构信息
Apoptosis Research Group, Heart Foundation Research Centre, School of Medical Science, Griffith University, Southport, Qld 9716, Australia.
出版信息
FEBS Lett. 2006 May 15;580(11):2671-6. doi: 10.1016/j.febslet.2006.04.019. Epub 2006 Apr 21.
Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by alpha-tocopheryl succinate (alpha-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were susceptible to alpha-TOS and less to TRAIL, while Met-5A cells were susceptible to TRAIL and resistant to alpha-TOS. MM cells expressed very low level of the Trx-1 protein, which was high in Met-5A cells, while the level of Trx-1 mRNA was similar in all cell lines. Downregulation of Trx-1 further sensitised Met-5A cells to TRAIL but not to alpha-TOS. Our data suggest that the role of Trx-1 in apoptosis modulation is unrelated to its anti-oxidant properties.
恶性间皮瘤（MM）是一种致命的癌症类型。我们研究了氧化还原活性蛋白硫氧还蛋白-1（Trx-1）在α-生育酚琥珀酸酯（α-TOS）和肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导MM细胞及其非恶性对应物（Met-5A）凋亡中的作用。MM细胞对α-TOS敏感，对TRAIL不敏感，而Met-5A细胞对TRAIL敏感，对α-TOS有抗性。MM细胞中Trx-1蛋白表达水平极低，在Met-5A细胞中则很高，而Trx-1 mRNA水平在所有细胞系中相似。Trx-1的下调进一步使Met-5A细胞对TRAIL敏感，但对α-TOS不敏感。我们的数据表明，Trx-1在凋亡调节中的作用与其抗氧化特性无关。
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